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antipcna antibody  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc antipcna antibody
    Antipcna Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1093 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/antipcna+antibody/PCNA+Mouse+mAb/pm41331243-356-38-40
    Average 96 stars, based on 1093 article reviews
    antipcna antibody - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    Blocking Assay:

    Article Title: Ultrasound-Triggered O2 Bombs: Perfluorobromooctane-Loaded Defect-Rich Metal-Organic framework for enhanced Sono-Immunotherapy
    Article Snippet: Sonodynamic therapy (SDT) has emerged as a promising strategy for treating tumors owing to its noninvasiveness and high tissue-penetrating depth.. Improving the efficiency of electron-hole separation of sonosensitizers and alleviation of the hypoxic tumor microenvironment remain major challenges.. Metal-organic frameworks (MOFs) have greatly potential in SDT application, however, exploring new sonosensitizers with high reactive oxygen species (ROS) generation and improving the efficacy through a synergistic effect in combination with other therapeutic modalities remain major challenges.

    Article Title: Wnt/β-Catenin Pathway Is Regulated by PITX2 Homeodomain Protein and Thus Contributes to the Proliferation of Human Ovarian Adenocarcinoma Cell, SKOV-3
    Article Snippet: .. The cells were kept in blocking solution (5% goat serum, 0.3% Triton X-100 in PBS) for 1 h, incubated with antiPCNA antibody (Cell Signaling Technology, Beverly, MA; dilution 1:100) for 2 h, followed by Alexa-Fluor 488-conjugated secondary antibody (Invitrogen; dilution 1:400) for 1 h. The cells were then stained with 1 mg/ml DAPI for 5 min and observed underNikonA1R confocalmicroscope usingNIS Element software. ..

    Incubation:

    Article Title: Ultrasound-Triggered O2 Bombs: Perfluorobromooctane-Loaded Defect-Rich Metal-Organic framework for enhanced Sono-Immunotherapy
    Article Snippet: Sonodynamic therapy (SDT) has emerged as a promising strategy for treating tumors owing to its noninvasiveness and high tissue-penetrating depth.. Improving the efficiency of electron-hole separation of sonosensitizers and alleviation of the hypoxic tumor microenvironment remain major challenges.. Metal-organic frameworks (MOFs) have greatly potential in SDT application, however, exploring new sonosensitizers with high reactive oxygen species (ROS) generation and improving the efficacy through a synergistic effect in combination with other therapeutic modalities remain major challenges.

    Article Title: Effects of reduced extracellular sodium on proliferation and invasive activity of renal cell carcinoma cell lines.
    Article Snippet: Thirty μg of proteins were separated in 10% or 12% Mini-PROTEAN TGX Stain-Free Precast Gels (Biorad, Hercules, CA, USA), transferred to a PVDF membrane using the InvitrogenTM Power Blotter System (15806162, Invitrogen Corporation, Waltham, MA, USA) and blocked in 5% milk. .. The membranes were then incubated with specific primary antibodies ON at 4 °C: anti-Akt antibody (9272S, 1:1000, Cell Signaling Technology, Danvers, MA, USA), anti-P-Akt antibody (4058S, 1:1000, Cell Signaling Technology), anti-p44/42 MAPK (Erk1/2) antibody (#9102, 1:1000, Cell Signaling Technology), anti- phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) antibody (#4370, 1:1000, Cell Signaling Technology), anti-cyclin D1 antibody (04-1151, 1:500, Millipore), antiPCNA antibody (#2586S, 1:1000, Cell Signaling Technology), anti-RhoA antibody (26C4, 1:500, Santa Cruz Biotechnology, Dallas, TX, USA), anti-ROCK1 antibody (sc-17794, 1:500, Santa Cruz Biotechnology), antiEzrin/Radixin/Moesin antibody (3142T, 1:1000, Cell Signaling Technology), anti-Phospho-Ezrin/Radixin/ Moesin antibody (#3149, 1:1000, Cell Signaling Technology), anti-Nrf2 antibody (sc-365949, 1:500, Santa Cruz Biotechnology), anti-HMOX-1 antibody (SAB2108676, 1:1000, Sigma Aldrich, Sant Louis, MO, USA) and anti-Bcl-2 antibody (sc-7382, 1:500, Santa Cruz Biotechnology). .. After two washes in PBS with 1% Tween, the membranes were incubated with the specific secondary antibody conjugated to horseradish peroxidase (#7076, anti-mouse IgG or #7074, anti-rabbit IgG, Cell Signaling Technology) for 1 h. Chemiluminescence images were acquired using a Bio-Rad ChemiDoc Imaging System (Biorad).

    Article Title: Erythrocyte-Based Biomimetic MOFs as a Triple Epigenetic Regulator for Enhancing Anti-Leukemia Immunity.
    Article Snippet: .. For IHC, the tissue sections were deparaffinized, rehydrated and subjected to antigen retrieval, and then incubated overnight with antiPCNA antibody (#12727, Cell Signaling Technology). .. The sections were imaged under a Nikon confocal fluorescence microscope.

    Article Title: Wnt/β-Catenin Pathway Is Regulated by PITX2 Homeodomain Protein and Thus Contributes to the Proliferation of Human Ovarian Adenocarcinoma Cell, SKOV-3
    Article Snippet: .. The cells were kept in blocking solution (5% goat serum, 0.3% Triton X-100 in PBS) for 1 h, incubated with antiPCNA antibody (Cell Signaling Technology, Beverly, MA; dilution 1:100) for 2 h, followed by Alexa-Fluor 488-conjugated secondary antibody (Invitrogen; dilution 1:400) for 1 h. The cells were then stained with 1 mg/ml DAPI for 5 min and observed underNikonA1R confocalmicroscope usingNIS Element software. ..

    Article Title: Beta-adrenergic receptor blocker propranolol triggers anti-tumor immunity and enhances irinotecan therapy in mice colorectal cancer.
    Article Snippet: Colorectal cancer (CRC) stands as the second leading cause of cancer-related deaths worldwide with limited available medicines.. While drug repurposing comes as a promising strategy for cancer treatment, we discovered that propranolol (Prop), a non-selective β1 and β2 adrenergic receptor blocker, significantly inhibited the development of subcutaneous CT26 CRC and AOM/DSS-induced CRC models.. The RNA-seq analysis highlighted the activated immune pathways after Prop treatment, with KEGG analysis enriched in T-cell differentiation.

    Article Title: Nuclear actin and DNA replication stress regulate telomere maintenance by telomerase.
    Article Snippet: .. Following this, the coverslips were blocked with ABDIL buffer (20mM Tris [pH 7.5], 2% BSA, 0.2% fish skin gelatin, 120mM NaCl, 0.1% Triton X-100, 0.1% sodium azide) in a humidified chamber at room temperature (RT) for 1 h. AntiPCNA antibody (Cell Signaling Technology, 2586T) was then diluted in ABDIL (1:1000) and added to coverslips, which were incubated overnight in a humidified chamber at 4 °C. .. Coverslips were washed with PBS for 5min three times, before incubating with Anti-mouse Alexa Fluor 488 (Thermo Fisher Scientific, A-11001) secondary antibody diluted in ABDIL (1:2000) for 1 h at RT in a humidified chamber.

    Article Title: <p>The Role Of Hepatic Stellate Cells In Promoting Liver Metastasis Of Colorectal Carcinoma</p>
    Article Snippet: .. The sections were then incubated overnight at 4oC with the antiPCNA antibody (Cell Signaling Technology, Danvers, MA, USA) followed by incubation for 30 mins at room temperature with a biotinylated ECL anti-rabbit IgG (GE Healthcare, Chalfont St. Giles, UK). .. The color was developed using the diaminobenzidine substrate (Roche Diagnostics, Mannheim, Germany), and the sections were counterstained with hematoxylin.

    Cell-Signaling:

    Article Title: Ultrasound-Triggered O2 Bombs: Perfluorobromooctane-Loaded Defect-Rich Metal-Organic framework for enhanced Sono-Immunotherapy
    Article Snippet: Sonodynamic therapy (SDT) has emerged as a promising strategy for treating tumors owing to its noninvasiveness and high tissue-penetrating depth.. Improving the efficiency of electron-hole separation of sonosensitizers and alleviation of the hypoxic tumor microenvironment remain major challenges.. Metal-organic frameworks (MOFs) have greatly potential in SDT application, however, exploring new sonosensitizers with high reactive oxygen species (ROS) generation and improving the efficacy through a synergistic effect in combination with other therapeutic modalities remain major challenges.

    Immunohistochemistry:

    Article Title: Erythrocyte-Based Biomimetic MOFs as a Triple Epigenetic Regulator for Enhancing Anti-Leukemia Immunity.
    Article Snippet: .. For IHC, the tissue sections were deparaffinized, rehydrated and subjected to antigen retrieval, and then incubated overnight with antiPCNA antibody (#12727, Cell Signaling Technology). .. The sections were imaged under a Nikon confocal fluorescence microscope.

    Article Title: Beta-adrenergic receptor blocker propranolol triggers anti-tumor immunity and enhances irinotecan therapy in mice colorectal cancer.
    Article Snippet: Colorectal cancer (CRC) stands as the second leading cause of cancer-related deaths worldwide with limited available medicines.. While drug repurposing comes as a promising strategy for cancer treatment, we discovered that propranolol (Prop), a non-selective β1 and β2 adrenergic receptor blocker, significantly inhibited the development of subcutaneous CT26 CRC and AOM/DSS-induced CRC models.. The RNA-seq analysis highlighted the activated immune pathways after Prop treatment, with KEGG analysis enriched in T-cell differentiation.

    Staining:

    Article Title: Wnt/β-Catenin Pathway Is Regulated by PITX2 Homeodomain Protein and Thus Contributes to the Proliferation of Human Ovarian Adenocarcinoma Cell, SKOV-3
    Article Snippet: .. The cells were kept in blocking solution (5% goat serum, 0.3% Triton X-100 in PBS) for 1 h, incubated with antiPCNA antibody (Cell Signaling Technology, Beverly, MA; dilution 1:100) for 2 h, followed by Alexa-Fluor 488-conjugated secondary antibody (Invitrogen; dilution 1:400) for 1 h. The cells were then stained with 1 mg/ml DAPI for 5 min and observed underNikonA1R confocalmicroscope usingNIS Element software. ..

    Software:

    Article Title: Wnt/β-Catenin Pathway Is Regulated by PITX2 Homeodomain Protein and Thus Contributes to the Proliferation of Human Ovarian Adenocarcinoma Cell, SKOV-3
    Article Snippet: .. The cells were kept in blocking solution (5% goat serum, 0.3% Triton X-100 in PBS) for 1 h, incubated with antiPCNA antibody (Cell Signaling Technology, Beverly, MA; dilution 1:100) for 2 h, followed by Alexa-Fluor 488-conjugated secondary antibody (Invitrogen; dilution 1:400) for 1 h. The cells were then stained with 1 mg/ml DAPI for 5 min and observed underNikonA1R confocalmicroscope usingNIS Element software. ..



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